English National Curriculum - Science

Culturing microorganisms

Mission Objectives

Describe how bacteria multiply

Describe how to prepare an uncontaminated culture using aseptic technique

Explain why bacteria are cultured at lower temperatures in schools

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Keywords

Words and meanings to learn

microorganism

a tiny living organism, such as bacteria, fungi or viruses, that is too small to see with the naked eye

incubation

keeping cultures at a suitable temperature for microorganisms to grow

culture

a group of microorganisms that are grown in or on a nutrient medium under controlled conditions

inoculating loop

a small tool used to transfer microorganisms to a culture medium

aseptic technique

a set of procedures used to prevent contamination of cultures or the environment by unwanted microorganisms

This lesson has been written by

Samantha Batch

Samantha is an accomplished science educator with over 15 years of leadership experience and a strong background in scientific analysis. She has a proven record of leading departments effectively, improving practice, and working collaboratively with stakeholders.

In her current work, Samantha embeds evidence-informed pedagogical principles such as retrieval practice, clear modelling, and effective sequencing to support high-quality curriculum design and professional development. She draws on assessment insight and common misconceptions to help teachers secure strong student understanding.

She is a former Head of Chemistry and Sixth Form Tutor at Ilkley Grammar School, with extensive Key Stage 4 and 5 experience, and an AQA examiner, giving her valuable insight into assessment standards and exam requirements.

In her spare time, Samantha enjoys playing the accordion with her local orchestra and has recently taken up cricket. 

Required Resources

  • Petri dishes with nutrient agar
  • Inoculating loops
  • Bunsen burners
  • Heatproof mats
  • Suspension of bacteria 
  • Sticky clear tape
  • Beaker of disinfectant

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